Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT
نویسندگان
چکیده
Subcutaneous immunotherapy (SCIT) is a classic form of allergen-specific that used to treat birch pollen induced allergic asthma. To investigate the underlying molecular mechanisms SCIT, we aimed profile lung samples explore changes in differential proteome before and after SCIT mice with Fresh lungs were collected from three groups female BALB/c mice: 1) control mice, 2) pollen-induced 3) SCIT. Tandem mass tag (TMT) labelling coupled liquid chromatography-tandem spectrometry (LC-MS/MS) was analyze mice. Ingenuity pathway analysis (IPA) Gene Ontology (GO) classification applied identify differentially expressed proteins (DEPs) crucial pathways. The screened DEPs validated by immunohistochemistry analysis. A total 317 upregulated 184 downregulated asthma group compared those group. In contrast, 639 (163 456 proteins) identified comparison Among DEPs, 277 returned similar levels as relative non-asthma condition. Bioinformatic revealed played significant role leukocyte extravasation signaling pathway. related importance
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2021
ISSN: ['1932-6203']
DOI: https://doi.org/10.1371/journal.pone.0258051